dna plasmid pcdna3 flag pten (Addgene inc)
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dna plasmid pcdna3 flag pten
Dna Plasmid Pcdna3 Flag Pten, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+flag+pten/pcDNA3-FLAG+PTEN+(Plasmid+%2378777)/pmc11598603-65-46-50
Average 93 stars, based on 12 article reviews
Dna Plasmid Pcdna3 Flag Pten, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+flag+pten/pcDNA3-FLAG+PTEN+(Plasmid+%2378777)/pmc11598603-65-46-50
Average 93 stars, based on 12 article reviews
dna plasmid pcdna3 flag pten - by Bioz Stars,
2026-09
93/100 stars
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Plasmid Preparation:Article Title: Exosomal miR-9 released from HIV Tat stimulated astrocytes mediates microglial migration Article Snippet: Plasmid and oligo transfection A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg pcDNA3-Flag PTEN (Addgene, Plasmid #78777, Cambridge, MA) / control plasmid or target protector by using the LipofectaminTM 3000 reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. .. Plasmid and oligo transfection A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg Article Title: Exosomal miR-9 released from HIV Tat stimulated astrocytes mediates microglial migration Article Snippet: A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg pcDNA3-Flag PTEN (Addgene, Plasmid #78777, Cambridge, MA) / control plasmid or target protector by using the LipofectaminTM 3000 reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. .. A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg Article Title: Investigating the role of Hedgehog/GLI1 signaling in glioblastoma cell response to temozolomide Article Snippet: RNA interference was performed using Dharmafect 1 (Dharmacon, Lafayette, CO) according to the manufacturer’s protocol to deliver 100 nM siRNA (Integrated DNA Technologies, Coralville, IA) to cells at approximately 50–60% confluence. .. Four hours post-transfection, media containing transfection reagents was removed and replaced with fresh media, and cells were incubated at 37° C, 5% CO 2 for 72 hours prior to continued experimentation. siRNA sequences used in this study are as follows (written 5′ to 3′): siGLI1 sense: CCA GGA AUU UGA CUC CCA ATT, siGLI1 antisense: UUG GGA GUC AAA UUC CUG GCT, siScramble (siScr) sense: GUG CAC CAA CGA CUU AUC ATT, siScr antisense: UGA UAA GUC GUU GGU GCA CT. Plasmid DNA transfections were performed using Mirus TransIT X2 (Mirus Bio LLC, Madison, WI) to deliver 25 ng/mL Article Title: ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes Article Snippet: .. The PH-RFP and 2XFYVE-GFP constructs contain a PH domain derived from Akt1 showing preferential binding to PI(3,4,5)P3 > PI(3,4,)P2 and a FYVE domain from HRS binding PI(3)P, respectively. Transfection:Article Title: Exosomal miR-9 released from HIV Tat stimulated astrocytes mediates microglial migration Article Snippet: Plasmid and oligo transfection A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg pcDNA3-Flag PTEN (Addgene, Plasmid #78777, Cambridge, MA) / control plasmid or target protector by using the LipofectaminTM 3000 reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. .. Plasmid and oligo transfection A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg Article Title: Exosomal miR-9 released from HIV Tat stimulated astrocytes mediates microglial migration Article Snippet: A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg pcDNA3-Flag PTEN (Addgene, Plasmid #78777, Cambridge, MA) / control plasmid or target protector by using the LipofectaminTM 3000 reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. .. A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg Article Title: Investigating the role of Hedgehog/GLI1 signaling in glioblastoma cell response to temozolomide Article Snippet: RNA interference was performed using Dharmafect 1 (Dharmacon, Lafayette, CO) according to the manufacturer’s protocol to deliver 100 nM siRNA (Integrated DNA Technologies, Coralville, IA) to cells at approximately 50–60% confluence. .. Four hours post-transfection, media containing transfection reagents was removed and replaced with fresh media, and cells were incubated at 37° C, 5% CO 2 for 72 hours prior to continued experimentation. siRNA sequences used in this study are as follows (written 5′ to 3′): siGLI1 sense: CCA GGA AUU UGA CUC CCA ATT, siGLI1 antisense: UUG GGA GUC AAA UUC CUG GCT, siScramble (siScr) sense: GUG CAC CAA CGA CUU AUC ATT, siScr antisense: UGA UAA GUC GUU GGU GCA CT. Plasmid DNA transfections were performed using Mirus TransIT X2 (Mirus Bio LLC, Madison, WI) to deliver 25 ng/mL Article Title: EIF4E Phosphorylation as a Mechanism of Resistance To mTOR and Androgen Receptor Inhibition in Advanced Prostate Cancer Article Snippet: .. The following plasmids were used in the transfections: pCDNA3 (Invitrogen, Carlsbad, CA), pRL—HCV—FL (kindly provided by Dr. Xinbin Chen, UC Davis School of Veterinary Medicine), Article Title: Metabolic Impact of MKP-2 Upregulation in Obesity Promotes Insulin Resistance and Fatty Liver Disease Article Snippet: .. Mouse embryonic fibroblasts (MEFs) derived from Mkp-2 +/+ and Mkp-2 −/− mice were cultured as described [ ] and transfected with Article Title: The Androgen Receptor is a negative regulator of eIF4E Phosphorylation at S209: Implications for the use of mTOR inhibitors in advanced prostate cancer Article Snippet: Cells were transiently transfected using Lipofectamine2000 (Invitrogen, Grand Island, NY) according to manufacturer’s specifications. .. The following plasmids were used in the transfections: pCDNA3 (Invitrogen, Carlsbad, CA), pRL-HCV-FL (kindly provided by Dr. Xinbin Chen, UC Davis School of Veterinary Medicine), Control:Article Title: Exosomal miR-9 released from HIV Tat stimulated astrocytes mediates microglial migration Article Snippet: Plasmid and oligo transfection A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg pcDNA3-Flag PTEN (Addgene, Plasmid #78777, Cambridge, MA) / control plasmid or target protector by using the LipofectaminTM 3000 reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. .. Plasmid and oligo transfection A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg Article Title: Exosomal miR-9 released from HIV Tat stimulated astrocytes mediates microglial migration Article Snippet: A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg pcDNA3-Flag PTEN (Addgene, Plasmid #78777, Cambridge, MA) / control plasmid or target protector by using the LipofectaminTM 3000 reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. .. A172 cells were transfected with 0.5μg pEF6.mCherry-TSG101 plasmid (Addgene, Plasmid #38318, Cambridge, MA) and BV-2 cells were transfected with 0.5μg Article Title: EIF4E Phosphorylation as a Mechanism of Resistance To mTOR and Androgen Receptor Inhibition in Advanced Prostate Cancer Article Snippet: .. The following plasmids were used in the transfections: pCDNA3 (Invitrogen, Carlsbad, CA), pRL—HCV—FL (kindly provided by Dr. Xinbin Chen, UC Davis School of Veterinary Medicine), Article Title: The Androgen Receptor is a negative regulator of eIF4E Phosphorylation at S209: Implications for the use of mTOR inhibitors in advanced prostate cancer Article Snippet: Cells were transiently transfected using Lipofectamine2000 (Invitrogen, Grand Island, NY) according to manufacturer’s specifications. .. The following plasmids were used in the transfections: pCDNA3 (Invitrogen, Carlsbad, CA), pRL-HCV-FL (kindly provided by Dr. Xinbin Chen, UC Davis School of Veterinary Medicine), Incubation:Article Title: Investigating the role of Hedgehog/GLI1 signaling in glioblastoma cell response to temozolomide Article Snippet: RNA interference was performed using Dharmafect 1 (Dharmacon, Lafayette, CO) according to the manufacturer’s protocol to deliver 100 nM siRNA (Integrated DNA Technologies, Coralville, IA) to cells at approximately 50–60% confluence. .. Four hours post-transfection, media containing transfection reagents was removed and replaced with fresh media, and cells were incubated at 37° C, 5% CO 2 for 72 hours prior to continued experimentation. siRNA sequences used in this study are as follows (written 5′ to 3′): siGLI1 sense: CCA GGA AUU UGA CUC CCA ATT, siGLI1 antisense: UUG GGA GUC AAA UUC CUG GCT, siScramble (siScr) sense: GUG CAC CAA CGA CUU AUC ATT, siScr antisense: UGA UAA GUC GUU GGU GCA CT. Plasmid DNA transfections were performed using Mirus TransIT X2 (Mirus Bio LLC, Madison, WI) to deliver 25 ng/mL Derivative Assay:Article Title: Metabolic Impact of MKP-2 Upregulation in Obesity Promotes Insulin Resistance and Fatty Liver Disease Article Snippet: .. Mouse embryonic fibroblasts (MEFs) derived from Mkp-2 +/+ and Mkp-2 −/− mice were cultured as described [ ] and transfected with Article Title: ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes Article Snippet: .. The PH-RFP and 2XFYVE-GFP constructs contain a PH domain derived from Akt1 showing preferential binding to PI(3,4,5)P3 > PI(3,4,)P2 and a FYVE domain from HRS binding PI(3)P, respectively. Cell Culture:Article Title: Metabolic Impact of MKP-2 Upregulation in Obesity Promotes Insulin Resistance and Fatty Liver Disease Article Snippet: .. Mouse embryonic fibroblasts (MEFs) derived from Mkp-2 +/+ and Mkp-2 −/− mice were cultured as described [ ] and transfected with Construct:Article Title: ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes Article Snippet: .. The PH-RFP and 2XFYVE-GFP constructs contain a PH domain derived from Akt1 showing preferential binding to PI(3,4,5)P3 > PI(3,4,)P2 and a FYVE domain from HRS binding PI(3)P, respectively. Binding Assay:Article Title: ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes Article Snippet: .. The PH-RFP and 2XFYVE-GFP constructs contain a PH domain derived from Akt1 showing preferential binding to PI(3,4,5)P3 > PI(3,4,)P2 and a FYVE domain from HRS binding PI(3)P, respectively. |